|
KMID : 0380220020350060609
|
|
Journal of Biochemistry and Molecular Biology
2002 Volume.35 No. 6 p.609 ~ p.614
|
|
|
The Anti-proliferative Gene TIS21 Is Involved in Osteoclast Differentiation
|
|
Lee Soo-Woong
Kwak Han-Bok
Lee Hong-Chan
Kim Hong-Hee
Lee Zang-Hee
|
|
|
Abstract
|
|
|
The remodeling process of bone is accompanied by
complex changes in the expression levels of various genes.
Several approaches have been employed to detect
differentially-expressed genes in regard to osteoclast
differentiation. In order to identify the genes that are
involved in osteoclast differentiation, we used a cDNAarray-nylon
membrane. Among 1,200 genes that showed a
measurable signal, 19 genes were chosen for further study.
Eleven genes were up-regulated; eight genes were downregulated.
TIS21 was one of the up-regulated genes which
were highly expressed in mature osteoclasts. To verify the
cDNA microarray results, we carried out RT-PCR and
real-time RT-PCR for the TIS21 gene. The TIS21 mRNA
level was higher in differentiated-osteoclasts when
compared to undifferentiated bone-marrow macrophages.
Furthermore, the treatment with 1 μM of a TIS21
antisense oligonucleotide reduced the formation of
osteoclasts from the bone-marrow-precursor cells by
~30%. These results provide evidence for the potential role
of TIS21 in the differentiation of osteoclasts.
|
|
|
KEYWORD
|
|
|
Antisense oligonucleotide, cDNA array TIS21
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|